Bioinformatics Seminar
Time: 11AM
Venue: Online
1 August 2023
Development of bioinformatics tools for analysing Oxford Nanopore RNA sequencing data
Yupei YouWEHI Bioinformatics
Oxford Nanopore sequencing produces long reads that have natural advantages for characterising the expression of genes and their different RNA products (called isoforms). However, the higher error rates in nanopore reads create difficulties when high accuracy is required, such as identification of RNA splice sites in RNA or the identification of cell barcodes in single-cell nanopore RNA sequencing data. To perform these tasks using error-prone nanopore data, existing methods mainly rely on extra short-read sequencing data or transcript annotations, limiting usability in many circumstances. In this talk, I will introduce the bioinformatics tools I have developed to perform these tasks solely using nanopore data and present the data analysis showing the benefits of using these tools.