Bioinformatics Seminar
Time: 3PM
Venue: Online
4 July 2023
A user-friendly R package for the implementation and assessment of RUVIII-PRPS performance in large-scale RNA sequencing data
Marie TrussartWEHI Bioinformatics
Large scale datasets generated by different omics technologies present unique challenges in terms of normalization and integration. I will be focusing on the RUV normalization methods, which have shown great promise in dealing with the challenges presented by large scale datasets from TCGA. RUVIII-PRPS is a novel strategy (Molania et al, 2023, Nat. Biotech, https://www.nature.com/articles/s41587-022-01440-w#code-availability) that uses pseudo-replicates of pseudo-samples (PRPS) to normalize RNA-seq data in situations. In this talk, I will be presenting the new user-friendly R package that we have been developing to enable researchers to run RUVIII-PRPS method and to visualize diagnostic plots before and after normalization to assess the quality and consistency of their data.