Bioinformatics Seminar
Time: 11AM
Venue: Davis Auditorium and Teams
15 November 2022
Understanding the mechanisms of gene regulation in B cells
Junli NieWEHI Bioinformatics
The B cell is the centre of humoral immunity. B cells derive from haematopoietic stem cells and differentiate through multiple stages, ultimately become antibody-secreting cells to generate protective antibodies against pathogens. The transition between the B cell stages relies on the dynamics of gene regulation in which DNA coding regions become accessible to RNA polymerases to synthesise RNA with the help of transcription factors and other factors. To dissect the changes in gene regulation in B cells, paired RNAseq and ATACseq data from virtually all stages of B cell differentiation was analysed. Based on that paired data, B cell trajectory and clustering was revealed, transcription factor-cis regulatory region-gene expression regulatory axes were established, and differential expressed genes and differential accessible regulatory DNA regions were identified. This analysis begins to reveal the regulatory patterns of gene regulation throughout B cell differentiation.