Bioinformatics Seminars

Bioinformatics Seminar

Time: 11AM
Venue: Teams

19 July 2022

Robust differential composition and variability analysis for multisample single-cell omics

Stefano Mangiola
WEHI Bioinformatics

Cell omics such as single-cell genomics, proteomics and microbiomics allow the characterisation of tissue and microbial community composition, which can be compared between conditions to identify biological drivers. This strategy has been critical to unveiling markers of disease progression, such as cancer and pathogen infection. No method for differential variability analysis exists for single-cell omic data, and methods for differential composition analysis only take a few fundamental data properties into account. Here we introduce sccomp, a generalised method for differential composition and variability analyses able to jointly model data count distribution, compositionality, group-specific variability and proportion mean-variability association, with awareness against outliers. Sccomp is an extensive analysis framework that allows realistic data simulation and cross-study knowledge transfer. We provide principles for differential variability analysis. We show that sccomp accurately fits experimental data, with a 50% incremental improvement over state-of-the-art algorithms. Using sccomp, we identified novel differential constraints and composition in the microenvironment of primary breast cancer.

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