Bioinformatics Seminar
Time: 11AM
Venue: Teams
31 May 2022
A computational perspective on the latest modalities of CRISPR/Cas gene editing systems
Goknur GinerWEHI Bioinformatics/Blood Cells and Blood Cancer
Cancer is one of the five leading causes of death in Australia and the genetic defects caused by point mutations are the cornerstone of cancer development. As a powerful application of genome editing, CRISPR/Cas systems quickly translated into new technologies for genome engineering in cell lines and living animals, unfolding critical cancer-driving and therapeutic resistance mechanisms. Moreover, one of the latest modalities of CRISPR/Cas systems, base editors, is the only way to correct point mutations without relying on cells' intervention, which often causes undesired permanent genetic alterations. My talk will illustrate the bioinformatic challenges in a few datasets from different CRISPR systems: CRISPR knockout, CRISPR activation, and CRISPR base editing. In addition to this, I will highlight our ongoing efforts to design efficient guides for base editors and to translate human mutations into a mouse that can be utilised in mouse genetic engineering models.