Methods for Immunological Analysis of ELISPOT data.

by Tim Beissbarth

Supplement for paper: "A systematic approach for comprehensive T-cell epitope discovery using peptide libraries."

Reference:
A systematic approach for comprehensive T-cell epitope discovery using peptide libraries.
Beissbarth T, Tye-Din JA, Smyth GK, Speed TP, Anderson RP
Bioinformatics; 7.2005; 21(ISMB Supplement 1), in press.
Source code: (knowledge of Perl and R required)
- Perl program to generate peptides from a group of proteins.
- R code to run EM algorithm on ELISPOT T-cell assay data.
Online Version of Tools:
- A program to generate all possible peptide epitopes from a group of proteins.
- Display matches of generated peptides versus individual sequences.
- Display matches of generated peptides versus multiple alignment.
- An EM algorithm to analyse data from an ELISPOT reader. Not yet available. Use R code.
  Estimates strength of responces (lambdas) and proportion of people responding to a peptide.

Address

Walter and Eliza Hall Institute of Medical Research
Tim Beißbarth, Bioinformatics
1G Royal Parade
Parkville 3050 VIC / Australia
Phone: +613 9345 2629
Fax: +613 9347 0852
Email: beissbarth@wehi.edu.au

Tim Beißbarth - Last Change: 15.03.05