Bioinformatics Seminar
Time: 11AM
Venue: Zoom Webinar
17 August 2021
Exploiting the full ribosomal RNA operon for microbiome profiling.
Chris WoodruffWEHI Bioinformatics
The identification of microbiota and their abundance in microbiome samples (profiling) is typically achieved using either shotgun whole metagenome sequencing or selection of specific marker genes - typically the ribosomal RNA (rrn) operon. Long read sequencing allows use of the full rrn operon, rather than the well-established use of sub-regions of the 16S small subunit of this operon. In this talk I will describe progress in exploiting the capabilities of error-prone longread sequencing for microbiome profiling. A particular focus is on the 23S large sub-unit, and the merging of information from different sub-regions of the rrn operon.