Bioinformatics Seminar
Time: 11AM
Venue: Davis Auditorium Hybrid
25 May 2021
Quantifying the contribution of time and division to B cells development
Ilariya TarasovaWEHI Bioinformatics
B cells, a type of lymphocyte, play a critical role in the adaptive immune system. They have a sophisticated decision-making mechanism of differentiation into different cell types. As the cells divide, levels in key regulatory molecules alter and guide the differentiation path of each individual cell. However, the mechanisms governing this process and how cells transit to new cell types are still poorly understood at the molecular level.
In my talk I will discuss how we are using RNA-seq to undertake a comprehensive inventory of which gene changes, occurring during B cell response programs, are affected by time (and not division), by division (and not time), and by a combination of time and division. We developed an intuitive statistical evaluation method to quantify the effect of our two variables and used it to identify dominating transcriptional regulators controlling the aforementioned patterns.