Bioinformatics Seminar
Time: 11:00am Tuesdays.
Venue:
Zoom Webinar
30 March 2021
Single cell transcriptome atlas of mouse and human mammary gland
Yunshun ChenWEHI Bioinformatics
Breast cancer has become the most diagnosed cancer in women. In the last decade we have been working on transcriptomic profiles of human and mouse mammary gland using the next generation sequencing platform. Now as the single-cell technology evolves, we are able to gain further insights into cellular diversity within the gland. In this talk, I will present two single cell transcriptome atlases of mouse and human mammary gland. For mouse, we profiled ~100,000 single cells from nine different developmental stages spanning late embryogenesis, early postnatal, prepuberty, adult, mid-pregnancy, late-pregnancy, and post-involution in order to understand how cellular diversity changes during mammary ontogeny. For human, we determined the single cell transcriptomes of ~430,000 cells encompassing normal breast, preneoplastic BRCA1+/- tissue, the major breast cancer subtypes, and pairs of tumours and involved lymph nodes (LN) to examine global changes in breast heterogeneity across different states. The two comprehensive cell atlases provide a high-resolution map of cell diversity within the mammary gland and represent valuable frameworks for future studies.