Bioinformatics Seminar
Time:
Venue: Na
29 September 2020
NaRemoving unwanted variation with CytofRUV to integrate multiple CyTOF datasets
Marie TrussartWEHI Bioinformatics
Mass cytometry (CyTOF) is a technology that has revolutionised single-cell biology. By detecting over 40 proteins on millions of single cells ; CyTOF allows the characterisation of cell subpopulations in unprecedented detail. However ; most CyTOF studies require the integration of data from multiple CyTOF batches usually acquired on different days and possibly at different sites. To date ; the integration of CyTOF datasets remains a challenge due to technical differences arising in multiple batches. To overcome this limitation ; we developed an approach called CytofRUV for analysing multiple CyTOF batches ; which includes an R-Shiny application with diagnostic plots. CytofRUV can correct for batch effects and integrate data from large numbers of patients and conditions across batches ; to confidently compare cellular changes and correlate these with clinically relevant outcomes.;Please note that this seminar will be held at 3pm;