Bioinformatics Seminar
Time:
Venue: Na
21 May 2019
NaGet your variants together! Call and combine time-course mutations from Leukemia RNA-Seq
Anna QuaglieriWEHI Bioinformatics
RNA Sequencing allows researchers to study the cancer genome from different perspectives than differential expression analyses. For example ; it is used to detect fusion genes ; differentially spliced genes and it can also be used to call mutations ; a key component in the study of cancer. In this talk ; I will discuss the strategies that I used to call variants from RNA-Seq samples and that were applied to two Leukemia cohorts to investigate relapse mechanisms. I will also discuss pros and cons that were found using different callers as well as strategies and R packages to analyse and explore time-course mutations using samples extracted from the same patient over the course of the treatment. I will conclude by showing an application to our cohort of Core Binding Factor Acute Myeloid Leukemia samples.;;;;