Bioinformatics Seminars

Bioinformatics Seminar

Time: 10:45am Tuesdays.
Level 7 Seminar Room 2, WEHI1

7 May 2019

Long-read single cell RNA sequencing with 10X and nanopore sequencing

Luyi Tian
WEHI Epigenetics & Development

Single cell RNA-seq (scRNA-seq) is rapidly gaining favour for understanding biology. Long-read methods have great potential for scRNA-seq by facilitating the identification and quantification of gene isoforms, allowing cell specific variation in isoform expression and splicing to be characterised. Previous methods for transcriptome-wide long-read scRNA-seq have been limited by low numbers of cells and/or few reads per cell. We have developed an improved Nanopore long-read scRNA-seq method that allows the profiling of hundreds of single-cells at high read-depth and demonstrate its use by profiling five human cancer cell lines. We then profiled muscle stem cells with and without activation and identified isoforms that associated with muscle stem cell activation and differentiation.

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