Bioinformatics Seminars

Bioinformatics Seminar

Time: 10:45am Tuesdays.
Venue:
Level 7 Seminar Room 2, WEHI1

19 June 2018

Actual location : Level 7 Boardroom

Examining differences in genome wide chromatin architecture with Hi-C: Differential analysis of B cell activation

Hannah Coughlan
WEHI Bioinformatics

Although chromatin is traditionally viewed in a linear sense, recently it has been recognised that the higher order organisation of the chromatin is extremely relevant to biological function by regulating gene expression and potentially cell fate. High throughput chromosome conformation capture techniques (Hi-C) can examine global chromatin architecture. However, interpretation of chromatin structure and understanding impact on gene expression is often difficult because of spatial resolution limitations.
Recent studies have established that cells have lineage-specific three-dimensional genome organization; however, it is unclear how such specific architecture is established or maintained. Using Hi-C, RNA-seq and ChIP-seq data, we show that the transcription factor Pax5 is critical for the global lineage-specific chromatin structure of B cells during activation. In our analysis of B cell activation, we used the differential analysis pipeline diffhic to identify regions of strengthening or weakening chromatin structure and associate the change with the lineage-defining transcription factor.


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