Bioinformatics Seminar
Time:
Venue: Na
19 June 2018
NaExamining differences in genome wide chromatin architecture with Hi-C: Differential analysis of B cell activation
Hannah CoughlanWEHI Bioinformatics
Although chromatin is traditionally viewed in a linear sense ; recently it has been recognised that the higher order organisation of the chromatin is extremely relevant to biological function by regulating gene expression and potentially cell fate. High throughput chromosome conformation capture techniques (Hi-C) can examine global chromatin architecture. However ; interpretation of chromatin structure and understanding impact on gene expression is often difficult because of spatial resolution limitations.
Recent studies have established that cells have lineage-specific three-dimensional genome organization; however ; it is unclear how such specific architecture is established or maintained. Using Hi-C ; RNA-seq and ChIP-seq data ; we show that the transcription factor Pax5 is critical for the global lineage-specific chromatin structure of B cells during activation. In our analysis of B cell activation ; we used the differential analysis pipeline diffhic to identify regions of strengthening or weakening chromatin structure and associate the change with the lineage-defining transcription factor.;Actual location : Level 7 Boardroom;