Bioinformatics Seminar
Time:
Venue: Na
5 December 2017
NaUsing ATAC-seq to study chromatin accessibility and gene regulation
Yang LiaoWEHI Bioinformatics
Changes on chromatin accessibility take an important part in the regulation of gene expression. ATAC-seq is proven to be useful in studying chromatin accessibility ; and many pipelines have been used in sequencing read analysis. By combining our short read aligner and read quantifier ; the Homer peak caller and the Limma/edgeR packages ; our pipeline provides a fast yet systematic view to the changes on chromatin accessibility between sample ; therefore helping biologists to understand the roles of the key transcription factors in gene expression regulation. In this talk ; I will briefly introduce the ATAC-seq technologies and its use in analyses ; then present our pipeline for studying the functions of key transcription factors in two real cases.;;