Bioinformatics Seminars

Bioinformatics Seminar

Time: 10:45am Tuesdays.
Level 7 Seminar Room 2, WEHI1

11 July 2017

Simultaneous variant calling and DNA base modification detection with nanopore sequencing

Scott Gigante
WEHI Bioinformatics

Nanopore sequencing is quickly emerging as a valuable alternative, and complement, to short-read NGS and other long-read technologies. One notable feature of nanopore sequencing is its capability to detect DNA base modifications, such as 5-methylcytosine, in untreated DNA. This allows whole-genome sequencing and modification detection in the same sequencing run. In this talk, I will examine the technical validity of nanopore-derived methylation calls by comparing these to matched reduced representation bisulfite sequencing, and extend the analysis of these data to examine differentially methylated regions in mice.

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