Bioinformatics Seminar
Time:
Venue: Na
11 July 2017
NaSimultaneous variant calling and DNA base modification detection with nanopore sequencing
Scott GiganteWEHI Bioinformatics
Nanopore sequencing is quickly emerging as a valuable alternative ; and complement ; to short-read NGS and other long-read technologies. One notable feature of nanopore sequencing is its capability to detect DNA base modifications ; such as 5-methylcytosine ; in untreated DNA. This allows whole-genome sequencing and modification detection in the same sequencing run. In this talk ; I will examine the technical validity of nanopore-derived methylation calls by comparing these to matched reduced representation bisulfite sequencing ; and extend the analysis of these data to examine differentially methylated regions in mice.
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