Time: 10:45am Tuesdays.
Level 7 Seminar Room 2, WEHI1
7 February 2017Visit the biography of the speaker
Chromosome Conformation in ContextJames Taylor
Johns Hopkins University
Chromosome conformation capture (3C) techniques have revealed many features about the structure of chromatin: Large scale organization into two compartments (A and B), relatively stable partitioning into Topologically Associating Domains (TADs) at the megabase scale, and more dynamic and cell type specific interactions associated with various architectural proteins at the sub-megabase scale (sub-TADs). However, these assays are limited in that they only interrogate interactions between chromatin without any connection to location in the nucleus, and are an ensemble measurement over thousands of cells. Here we describe integrated analysis of chromosome conformation capture (Hi-C) data, DamID, and a novel single cell chromatin paint approach to understand the relationship of chromosome conformation features with a specific nuclear compartment, the periphery.