Bioinformatics Seminars

Bioinformatics Seminar

Time: 10:45am Tuesdays.
Venue:
Level 7 Seminar Room 2, WEHI1

7 February 2017

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Chromosome Conformation in Context

James Taylor
Johns Hopkins University

Chromosome conformation capture (3C) techniques have revealed many features about the structure of chromatin: Large scale organization into two compartments (A and B), relatively stable partitioning into Topologically Associating Domains (TADs) at the megabase scale, and more dynamic and cell type specific interactions associated with various architectural proteins at the sub-megabase scale (sub-TADs). However, these assays are limited in that they only interrogate interactions between chromatin without any connection to location in the nucleus, and are an ensemble measurement over thousands of cells. Here we describe integrated analysis of chromosome conformation capture (Hi-C) data, DamID, and a novel single cell chromatin paint approach to understand the relationship of chromosome conformation features with a specific nuclear compartment, the periphery.


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