Bioinformatics Seminar

Time:
Venue: Na

7 February 2017

Chromosome Conformation in Context

James Taylor
Johns Hopkins University

Chromosome conformation capture (3C) techniques have revealed many features about the structure of chromatin: Large scale organization into two compartments (A and B) ; relatively stable partitioning into Topologically Associating Domains (TADs) at the megabase scale ; and more dynamic and cell type specific interactions associated with various architectural proteins at the sub-megabase scale (sub-TADs). However ; these assays are limited in that they only interrogate interactions between chromatin without any connection to location in the nucleus ; and are an ensemble measurement over thousands of cells. Here we describe integrated analysis of chromosome conformation capture (Hi-C) data ; DamID ; and a novel single cell chromatin paint approach to understand the relationship of chromosome conformation features with a specific nuclear compartment ; the periphery.;Visit the biography of the speaker

The Walter and Eliza Hall Institute of Medical Research