Bioinformatics Seminar
Time:
Venue: Na
15 November 2016
NaRsubread: ultrafast read mapping and quantification for RNA and DNA sequencing data
Wei ShiWEHI Bioinformatics
Rsubread is a Bioconductor R package that encompasses multiple tools for fast and accurate analysis of next-generation sequencing data. Major functionalities of this toolbox include mapping of RNA and DNA sequencing reads to a reference genome ; counting reads to genomic features such as genes ; exons ; junctions and genomic intervals (quantification) and discovery of genomic mutations. In this talk ; I will describe our recent improvement on the mapping of multi-mapping reads and also new features that we have implemented for the quantification of genomic features. I will also present some results of comparing Rsubread to other software tools for the mapping and quantification of sequence reads. Read mapping and quantification is an essential step in most sequence data analyses. I will show that Rsubread achieves a much higher computational efficiency than other analysis pipelines. It is also found to achieve comparable or higher accuracy in mapping and quantification.;;;;