Bioinformatics Seminar
Time:
Venue: Na
2 August 2016
NaDiscovering Protein-Protein Interactions from DNA sequence - insights into the cardiac gene regulatory network and disease
Ashley WaardenbergThe University of Sydney
NKX2-5 is a key transcription factor (TF) required for normal heart development and is implicated in a range of cardiac diseases. NKX2-5 is a critical TF for normal heart development that binds directly to DNA by recognising a specific sequence called the NKX2-5 binding element (NKE). However ; until recently its genomic targets were poorly defined and the NKX2-5 protein-protein interaction network remains poorly defined. Recently we identified genomic target regions for NKX2-5 and human disease relevant mutations in cultured HL-1 cardiomyocytes using the DamID method and identified new NKX2-5 disease mechanisms (Bouveret R ; Waardenberg AJ ; et al. eLIFE ; 2015). Here ; we predict and subsequently validate completely novel NKX2-5 PPIs based on recurrent binding sites through the application of machine learning algorithms.
;;;